主要研究领域:昆虫RNAi机理研究及应用;肠道微生物资源开发与利用
主要讲授课程:《分子生物学》,《昆虫与人类》
联系方式:18221070887;E-mail: guanruobing@126.com
一、承担及完成的主要科研项目
1. 国家自然科学基金青年项目,dsRNA在昆虫体内的剪切模式研究,31702057,2018.01-2020.12,主持,结题
2. 中国博士后科学基金面上项目,鳞翅目昆虫特异up56基因影响RNAi效率的机理研究,2016M601672,2016.11-2018.10,主持,结题
3. 伟德BETVLCTOR1946博士科研启动项目,30500755,2019.06-2021.06,主持,结题
4. 河南省青年托举人才工程项目,鳞翅目昆虫特异核酸酶对RNAi通路的作用机理研究,2020HYTP038,2019.1-2021.12,主持,结题
5. 河南省高等学校重点科研项目,影响亚洲玉米螟 RNAi 效率的核酸酶研究, 21A210017,2021.1-2022.12,主持,结题
6. 河南省重点研发与推广专项(科技攻关),棉铃虫RNA干扰杀虫剂的开发与应用,212102110442,2021.1-2022.12,主持,结题
7. 中国科学院昆虫发育与进化生物学重点实验室开放课题,棉铃虫肠道共生菌H7对RNAi通路影响机理研究,2021.1-2022.12,主持,结题
8. 上海市科技兴农项目,蔬菜害虫干扰剂的研究与创制,2019-02-08-00-12-F01125,2019.4.19-2022.4.15,参与,结题
9. 上海市科技兴农项目,防治重要蔬菜害虫黄曲条跳甲的生物农药创制与应用,参与,2022-02-08-00-12-F01125,2022.4.01-2025.3.31,参与,在研
二、 发表的主要学术论文
1. Ruobing Guan#, Shaoru Hu#, Xiang Li, Shiheng An, Xuexia Miao, Haichao Li, A
TIL-Type serine protease inhibitor involved in humoral immune response of Asian Corn Borer Ostrinia furnaculis, Frontiers in Immunology, 2022, 13:900129. (IF: 8.787)
2. Haichao Li, Yongjun Wei*, Guohui Yuan, Ruobing Guan*(通讯作者), Insight into
maize gene expression profiles responses to symbiotic bacteria derived from Helicoverpa armigera and Ostrinia furnacalis, Archives of Microbiology, 2022, 204:56. (IF: 2.667)
3. Ruobing Guan, Dongdong Chu, Xinyi Han, Xuexia Miao*, Haichao Li*, Advances
in the development of microbial double-stranded RNA production systems for application of RNA interference in agricultural pest control, Frontiers in Bioengineering and Biotechnology, 2021, 9:753790. (IF: 6.064)
4. Tong Li#, Ruobing Guan#, Yuqing Wu*, Su Chen, Guohui Yuan, Xuexia Miao,
Haichao Li*, The Novel Agrotis ipsilon Nora Virus Confers Deleterious Effects to the Fitness of Spodoptera frugiperda (Lepidoptera: Noctuidae), Frontiers in Microbiology, 2021, 12:727202. (IF: 6.064)
5. Shuangyan Yao, Yue Yang, Yuying Xue, Wenli Zhao, Xiaoguang Liu, Mengfang Du, Xinming Yin, Ruobing Guan*(通讯作者), Jizhen Wei*, Shiheng An*, New insights on the effects of spinosad on the development of Helicoverpa armigera. Ecotoxicology and Environmental Safety, 2021, 221:112452. (IF: 7.129)
6. Haichao Li, Yongjun Wei*, Guohui Yuan, Ruobing Guan*(通讯作者), Insight into maize gene expression profiles responses to symbiotic bacteria derived from Helicoverpa armigera and Ostrinia furnalis, Archives of Microbiology, 2021, 204(1):56. (IF: 2.552)
7. Ruobing Guan#*, Mengge Wang#, Zhonghua Guan, Cheng-Yun Jin, Wei Lin, Xiaojun Ji, Yongjun Wei*, Metabolic engineering for glycyrrhetinic acid production in Saccharomyces cerevisiae, Frontiers in Bioengineering and Biotechnology, 2020, 8:588255, (IF: 5.890)
8. Su Chen, Haichao Li, Guohui Yuan, Ruobing Guan*(通讯作者), Tong Li*, Complete genome sequence of a novel rhabdo like virus from the Chinese black cutworm Agrotis ipsilon (Lepidoptera: Noctuidae), Archives of Virology, 2020, 165(4):989-991, (IF: 2.243)
9. Ruobing Guan, Haichao Li, Hao Zhang, Shiheng An, Comparative analysis of dsRNA-induced lncRNAs in three kinds of insect species, Archives of Insect Biochemistry and Physiology, 2019, e21640, (IF: 1.698)
10. Ruobing Guan#*, Qiuyan Chen#, Haichao Li, Shaoru Hu, Xuexia Miao, Guirong Wang, Bin Yang*, Knockout of the HaREase gene improves the stability of dsRNA and increases the sensitivity of Helicoverpa armigera to Bacillus thuringiensis toxin, Frontiers in Physiology, 2019, 10:1368, (IF: 3.450)
11. Ruobing Guan#, Haichao Li#, Yujie Fan, Shaoru Hu, Olivier Christiaens, Guy Smagghe, Xuexia Miao*. A nuclease specific to lepidopteran insects suppresses RNAi. Journal of Biological Chemistry. 2018, 293(16):6011-6021. (IF:4.106)
12. Ruobing Guan#, Shaoru Hu#, Haichao Li, Zhenying Shi, Xuexia Miao*. The in vivo dsRNA cleavage has sequence preference in insects. Frontiers in Physiology. 2018, 9:17687, (IF:3.201)
13. Ruobing Guan, Haichao Li, Xuexia Miao*. Prediction of effective RNA interference targets and pathway-related genes in lepidopteran insects by RNA sequencing analysis. Insect Science. 2017, 00: 1-12. (IF:2.710)
14. Ruobing Guan, Haichao Li, Xuexiao Miao*. RNAi pest control and enhanced BT insecticidal efficiency achieved by dsRNA of chymotrypsin-like genes in Ostrinia furnacalis. Journal of Pest Science. 2016, 90(2):745-757. (IF:4.402)
15. Haichao Li, Ruobing Guan, Huimin Guo, Xuexia Miao*. New Insights into an RNAi approach for plant defense against piercing-sucking and stem-borer insect pests. Plant Cell and Environment. 2015, 38(11):2277-2285. (IF:6.96)
16. Hao Zhang, Haichao Li, Ruobing Guan, Xuexia Miao*. Lepidopteran insect species-specific, broad-spectrum, and systemic RNA interference by spraying dsRNA on Larvae. Entomologia Experimentalis Et Applicata. 2015, 155(3):218-228. (IF:1.616)
17. Haichao Li, Zhang Hao, Ruobing Guan, Xuexia Miao*. Identification of differential expression genes associated with host selection and adaptation between two sibling insect species by transcriptional profile analysis. BMC Genomics. 2013, 14:582. (IF:4.397)
18. 关若冰,李海超,苗雪霞,RNA生物农药的商业化现状及存在问题分析,中国农业科学,2022, 55(15):2949-2960.
19. 李祥,刘龙,安世恒,关若冰*(通讯作者),microRNA研究概况及其在农业生产中的应用前景,南方农业学报,2022,222,53(7):1832-1843.
20. 李海超,谢飞,张园琦,关若冰*(通讯作者),不同抗、感水稻品种对褐飞虱肠道菌群的影响,生物技术通报,2021,37(3):10-18.
21. 胡少茹,关若冰,李海超,苗雪霞,RNAi在农业防治中应用的重要进展及存在问题,昆虫学报,2019, 62(4):506-515.
22. 关若冰,赵文丽,刘晓光,安世恒,苏丽娟,农科类专业《分子生物学》课程教学改革探析,现代农业科技,2020年15期
三、出版的主要论著及教材
1.《RNA干扰:从基因功能到生物农药》,2021年,科学出版社,参编
四、主要授权专利
1. 魏纪珍,安世恒,席玉强,刘晓光,杜孟芳,关若冰,一种Bt蛋白防治棉铃虫的增效剂及其与Bt蛋白联合使用防治棉铃虫的方法,授权公告号:CN109874798B,授权时间:2021年3月23日
2. 苗雪霞,李海超,关若冰,一种提高dsRNA杀虫效果的配方,国内申请号:202010020939.X,时间:2020年01月09日
3. 刘晓光,关若冰,杨朔,安世恒,魏纪珍,利用环孢素A防治斜纹夜蛾的方法,授权时间:2020年10月02日,授权公告号:CN110692641B
4. 苗雪霞,李海超,关若冰,对蚜虫高效致死的RNAi靶标基因及其应用,国内申请号:201810455499.3,时间:2018年05月15日
5. 苗雪霞,关若冰,李海超,胡少茹,一种提高鳞翅目昆虫RNAi效率的基因及其应用,国内申请号:201611244499.6,时间:2016年12月30日
6. 苗雪霞,李海超,关若冰,郭惠民,一种通过灌溉防治害虫的RNAi技术及其应用,国际申请号:PCT/CN2014/090946;国内申请号:201310561150.5,时间:2015年05月20日